Reagent kit for the quantitative determination of Total & Direct Bilirubin based on Jendrassik & Grof’s method.
PRINCIPLE Bilirubin reacts with diazotized sulphanilic acid to form an azocompound the colour of which is measured at 546 nm (530-560 nm) and is proportional to the concentration of bilirubin. For total bilirubin reaction is accelerated by DCA(2,4- Dichloro Aniline) . The readings for total & direct bilirubin are taken after 5 minutes of incubation .
SAMPLE COLLECTION STORAGE & STABILITY Serum is the preferred specimen . Plasma with heparin as anticoagulant may b e used. Serum or Plasma should be separated as early as possible. (Samples are stable for a day when stored tightly capped at 2°c – 8°c and for a month a t – 10°c. Avoid exposure of s ample s to direct light during processing & storage. Gross contamination at any stage makes the samples un suitable for use. The samples should be brought to room temperature prior to use. Do not use hemolysed /grossly contaminated samples
